Chris Pratt Mario (voice) Anya Taylor-Joy Princess Peach (voice) Charlie Day Luigi (voice) Jack Black Bowser (voice) Keegan-Michael Key Toad (voice) Seth Rogen Donkey Kong (voice) Fred Armisen Cranky Kong (voice) Kevin Michael Richardson Kamek (voice) Sebastian Maniscalco Spike (voice) Khary Payton Penguin King (voice) Eric Bauza General Toad (voice) Jessica DiCicco Mario's Mom (voice) Jeannie Elias Plumbing Commercial Lady (voice) Rino Romano Uncle Tony (voice) John DiMaggio Uncle Arthur (voice) Scott Menville Koopa General (voice) Charles Martinet Mario's Dad / Giuseppe (voice) Carlos Alazraqui Additional Voices (voice) Ashly Burch Additional Voices (voice) Rachel Butera Additional Voices (voice) Cathy Cavadini Additional Voices (voice) Willow Geer Additional Voices (voice) Aaron Hendry Additional Voices (voice) Andy Hirsch Additional Voices (voice) Phil LaMarr Additional Voices (voice) Jeremy Maxwell Additional Voices (voice) Lee Shorten Additional Voices (voice) Cree Summer Additional Voices (voice)