Shameik Moore Miles Morales / Spider-Man (voice) Hailee Steinfeld Gwen Stacy / Spider-Woman (voice) Brian Tyree Henry Jeff Morales (voice) Luna Lauren Velez Rio Morales (voice) Jake Johnson Peter B. Parker / Spider-Man (voice) Oscar Isaac Miguel O'Hara / Spider-Man 2099 (voice) Jason Schwartzman Jonathan Ohnn / The Spot (voice) Issa Rae Jessica Drew / Spider-Woman (voice) Daniel Kaluuya Hobie Brown / Spider-Punk (voice) Karan Soni Pavitr Prabhakar / Spider-Man India (voice) Shea Whigham George Stacy (voice) Greta Lee LYLA (voice) Mahershala Ali Uncle Aaron / Prowler (voice) Amandla Stenberg Margo Kess / Spider-Byte (voice) Jharrel Jerome Miles G. Morales (voice) Andy Samberg Ben Reilly / Scarlet Spider (voice) Jack Quaid Peter Parker / The Lizard - Earth 65 (Voice) Rachel Dratch Ms. Weber (voice) Ziggy Marley Lenny (voice) Jorma Taccone Adriano Tumino / Medieval Vulture / '67 Spider-Man (voice) J.K. Simmons J. Jonah Jameson (voice) Donald Glover Aaron Davis / Prowler Elizabeth Perkins May / Quippy Spider-Person (voice) Kathryn Hahn Doc Ock (voice) Ayo Edebiri Glory (voice) Atsuko Okatsuka Yuri (voice) Peter Sohn Ganke (voice) Melissa Sturm Mary Jane Parker (voice) / Mayday Parker (voice) Taran Killam Web-Slinger (voice) Metro Boomin Metro Spider-Man (voice) Josh Keaton Spectacular Spider-Man (voice) Sofia Barclay Malala Windsor / Spider-UK (voice) Danielle Perez Charlotte Webber / Sun-Spider (voice) Yuri Lowenthal Insomniac Spider-Man (voice) Ismail Bashey Additional Voices (voice) Oscar Camacho Additional Voices (voice) David Michie Additional Voices (voice) Juan Pacheco Additional Voices (voice) Chrystee Pharris Additional Voices (voice) Ben Pronsky Additional Voices (voice) Al Rodrigo Additional Voices (voice) Jaswant Dev Shrestha Additional Voices (voice) Ruth Zalduondo Additional Voices (voice) Deepti Gupta Additional Voices (voice) Christopher Miller Additional Voices (voice) Andrea Navedo Additional Voices (voice) Jacqueline Pinol Additional Voices (voice) Amanda Troop Additional Voices (voice) June Christopher Additional Voices (voice) Marabina Jaimes Additional Voices (voice) Rez Kempton Additional Voices (voice) Lex Lang Additional Voices (voice) Michelle Ruff Additional Voices (voice) Jorge R. Gutierrez Additional Voices (voice) Kimiko Glenn Peni Parker (voice) (uncredited) Peggy Lu Mrs. Chen (uncredited) John Mulaney Peter Porker / Spider-Ham (voice) (archive footage) (uncredited) Andrew Garfield Peter Parker / Spider-Man (archive footage) (uncredited) Denis Leary Captain Stacy (archive footage) (uncredited) Tobey Maguire Peter Parker / Spider-Man (archive footage) (uncredited) Cliff Robertson Ben Parker (archive footage) (uncredited) Martin Sheen Ben Parker (archive footage) (uncredited) Alfred Molina Doctor Octopus (voice) (archive footage) (uncredited) Post Malone Bystander (voice) (archive footage) (uncredited) Jharrel Jerome Miles Morales / The Prowler (voice) (uncredited)